Revealing the Mechanisms of Zhen-Wu-Huang-Qi Decoction in the treatment of HFrEF

Integrating Network Pharmacology, Multi-Omics, Causal Inference & Molecular Docking

Authors
Affiliations

Kun Hou

Health Science Center, Xi’an Jiaotong University

Hanzhong Traditional Chinese Medicine Hospital

Supervisor’s name

Health Science Center, Xi’an Jiaotong University

The First Affiliated Hospital of Xi’an Jiaotong University

1 Project Overview

This project systematically explores the pharmacological mechanism of Zhen-Wu-Huang-Qi Decoction (ZWHQD) in the treatment of Chronic heart failure with reduced ejection fraction (HFrEF), based on network pharmacology, multi-omics bioinformatics, and computational verification.

The analytical pipeline integrates active component screening, target prediction, GEO transcriptome analysis, WGCNA co-expression network, PPI hub gene identification, functional enrichment, immune infiltration, single-cell transcriptomics, Mendelian randomization, colocalization causal inference, diagnostic model construction, single-gene GSEA and molecular docking.

2 Research Workflow

The full technical scheme and modular analysis pipeline are presented in Methods.